A new field leishmania test has been made, tested and evaluated in the “PLOS Neglected Tropical Diseases”. This brand-new test has been created by Laura Willen from the Charles University, Czech Republic. She and her colleagues developed an immunochromatographic test (ICT) that is able to rapidly screen the dogs for the presence of Phlebotomus perniciosus aka the Sand Fly. The test is supposed to detect the antibodies in the dog’s blood against one salivary protein from the sand fly, the SP03B. Detection of this protein is already done in laboratory conditions with an ELISA test. The new field test is planned to provide the same results as an ELISA test but faster and out of the laboratory.
The test has been tested and optimized with the help of 53 laboratory-bred Beagles that at some point were or were not exposed to 200 Phlebotomus perniciosus sand flies.
After the optimization, the team compared the results of the field test with ELISA results and they were nearly 100% compatible. This means that the new ICT test has 100% sensitivity and 86.79% specificity. Now the goal is to raise the specificity of the test to 96.23% without messing with the sensitivity percentage.
The new ICT test is designed to be easily operated without additional specialized equipment or special training. Further field detection accuracy is necessary to determine the exposure of the canine population to the sand flies and to properly validate the test.
Read the original article “Field test for dog Leishmania exposure evaluated“.
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